[Fwd: Re: Growing Arabidopsis in sand]

Wendy Allan wallan at uoguelph.ca
Thu May 3 09:05:25 EST 2001



-------- Original Message --------
Subject: Re: Growing Arabidopsis in sand
Date: Wed, 2 May 2001 10:11:50 -0700
From: Anne Britt <abbritt at ucdavis.edu>
To: wallan at uoguelph.ca (Wendy Allan)
References: <3AEEFB26.EFA193B8 at uoguelph.ca>

You might try vermiculite instead.  Another option is a courser grain
of sand, that won't pack around the roots so tightly.  Also, an open
system (like  pot, with drainage) might help with the algae- it
sounds like your plants are too wet- the roots might even be anoxic.
Good luck!


>Hello:
>
>     I am trying to grow Arabidopsis cv columbia in sand. I want to be
>able to quickly and easily harvest  the roots for analysis. Anyways, the
>plants are germinated and grown in deep-dish petri plates filled with
>moist sand under short-day conditions in a growth chamber. I fertilize
>the plants frequently (ie approx. every other day) with Hoaglund's
>solution. The petri plates are covered.  I am having trouble with algae
>growth. Also, I want the rosettes to grow large. So far, after 2 to 3
>weeks, the rosettes become completely chlorotic. I am also growing about
>10-12 plants per plate.
>
>    Does anyone have any suggestions as to how I can keep my plants
>healthy and thriving under this system? As well, for large rosettes, how
>long can I keep the plants growing?
>
>Wendy Allan,
>
>---

Anne Bagg Britt
Associate Professor of Plant Biology
University of California, Davis
Davis, CA 95616
fax: (530) 752-5410
phone: (530) 752-0699

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