[Fwd: Binary vector dependent unintended misexpression]

Nobody nobody at hgmp.mrc.ac.uk
Wed Nov 21 04:57:07 EST 2001


I don't remember my pCambia's, but my guess would be read through from some
other promoter upstream, possibly 35S, as I think they all have that.
Terminators are not really terminators, but just 'braking zones' for the RNA
polymerase to fall off, and a lot of the artificial ones are very short.  It
is noteworthy that pBIN19 effectively has a very long terminator after the
nos::kan gene, due to its numerous erroneous insertions - which happen to
enhance function.

If the added gene had a very long promoter fragment, it might help the RNA
polymerase from the previous gene fall off (is it very short?), but a better
be to would be to add an extra spacer element of some sort.  Not less that 5=
bp would be my guess.  Or just use another vector...!


Trevor Fenning.

Jim Tokuhisa wrote:

>=20 interesting!  Any ideas what might be happening.
>=20 --
>=20 Jim Tokuhisa, Ph.D.
>=20 E-mail:  tokuhisa at ice.mpg.de
>=20 Until 23 Nov 2001
>=20 Max Planck Institut f=FCr Chemische =D6kologie
>=20 Carl-Zeiss-Promenade 10
>=20 D-07745 Jena
>=20 Germany
>=20 Phone:  +49-3641-64-36-51
>=20 FAX:  +49-3641-64-36-50
>=20 >From 26 Nov 2001
>=20 Max Planck Institut f=FCr Chemische =D6kologie
>=20 Winzerlaer Strasse 10
>=20 D-07745 Jena
>=20 Germany
>=20 Phone:  +49-3641-57-1318
>=20 FAX:  +49-3641-57-1302
>=20   ---------------------------------------------------------------------=
>=20    Part 1.2    Type: Outlook Express Mail Message (message/rfc822)
>=20            Encoding: 7bit

Content-Type: text/x-vcard; charset=3Dus-ascii;
=20 name=3D"Fenning.vcf"
Content-Transfer-Encoding: 7bit
Content-Description: Card for Trevor Fenning
Content-Disposition: attachment;
=20 filename=3D"Fenning.vcf"

More information about the Arab-gen mailing list