RNA extraction with hot phenol

Alessia Para alessia.para at sh.se
Mon Oct 15 12:10:03 EST 2001


<x-charset ISO-8859-1>Hi everybody!
I'm trying to do a large scale RNA extraction from Arabidopsis seedlings
using the hot phenol protocol described in Verwoerd et al, NAR (1989): do
you have any suggestion for scaling up the volumes they indicate,
expecially the mixture extraction buffer-phenol, for something like 2-3 g
of tissue?
They refer to a paper by Van Slogteren et al., PMB (1983)but it's too old
and I can't find it: does anybody have it?
Thank you.

Alessia

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