Postdoc - pollen receptor kinases

Sheila McCormick sheilamc at nature.berkeley.edu
Mon Sep 17 13:19:10 EST 2001


Postdoc Position - Signaling via pollen receptor kinases, Plant Gene 
Expression Center, UC-Berkeley, Albany, CA
We are studying the signaling pathways mediated by pollen-specific 
receptor-like kinases in tomato (Plant Cell 10:319-330, 1998). In 
recent work, we have used the yeast two-hybrid system to identify 
ligands and downstream protein partners for these kinases. We have 
confirmed one of the ligand-kinase interactions using 
co-immunoprecipitation assays (see Abstract from ASPP mini-symposium 
at
http://www.rycomusa.com/aspp2001/public/M14/0953.html), and we have 
confirmed the interaction between the kinases and one downstream 
component, a potentially highly phosphorylated but otherwise 
uncharacterized protein, KPP1 (kinase partner protein). A 2nd round 
Y2H screen with KPP revealed potential interactions with cytoplasmic 
components already implicated in pollen tube growth. The postdoctoral 
fellow will carry out experiments to decipher the downstream 
components of the signaling pathway(s), including pulldown assays, 
deletion analyses and phenotypic and biochemical analyses of T-DNA 
insertions in the Arabidopsis homologs of the kinase partner 
proteins. Training and a publication record in biochemistry and 
molecular biology is required.  Prior experience with reproductive 
biology and/or kinases is a plus, but prior experience with plants is 
not necessary. Familiarity with bioinformatics is desirable. Funding 
is available for at least 2 years, but the potential for obtaining 
partial salary support via fellowship applications will be considered 
in applicant selection. Position available now, although a later 
start date can be negotiated; open until filled. Salary ~$30K. For 
consideration send a CV with a list of publications, a short 
statement of research interests and the names and
e-mail addresses and/or phone numbers of 3 references to: 
sheilamc at nature.berkeley.edu or to Sheila McCormick, Plant Gene 
Expression Center, 800 Buchanan St., Albany, CA 94710; FAX 
(510)-559-5678
For more information about the other projects in the lab, see 
http://www.pgec.usda.gov/McCormick/mclab.html



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