post-doctoral position available

Karen Browning kbrowning at
Tue Aug 20 10:45:58 EST 2002

Postdoctoral Position
The University of Texas at Austin, Department of Chemistry and 
Biochemistry and The Institute for Cellular and Molecular Biology

A postdoctoral position is available to study the role of the isozyme 
form of eIF4F in plants.  This NSF funded postdoctoral position will 
include biochemical analysis, insertion mutants, DNA arrays and gene 
silencing in Arabidopsis thaliana.

The initiation of protein synthesis in all eukaryotes is an intricate 
process requiring at least 8-10 known initiation factors.  The 
long-term goal of this laboratory is to elucidate a detailed 
molecular description of this process in plants.    Plant translation 
utilizes a second form of eIF4F not found in other eukaryotes.  This 
isozyme form is a complex composed of a cap-binding protein 
(eIF(iso)4E) and a large subunit eIF(iso)4G.  The analysis of the 
roles of these two forms of eIF4F will include the formation of mixed 
complexes of the subunits of eIF4F and eIF(iso)4F (i.e., 
eIF4G/eIF(iso)4E and eIF(iso)4G/eIF4E).  A genomic approach will also 
be taken that includes gene disruption of the subunits using T-DNA 
knockouts or gene silencing.  A DNA chip will be prepared that 
contains the genes for all the protein synthesis initiation, 
elongation and termination factors from A. thaliana.  This chip will 
be probed with RNAs obtained from various plant tissues, 
developmental stages and environmental conditions to determine which 
genes for eIF(iso)4F or eIF4F, or other protein synthesis factor 
genes, are regulated developmentally or during environmental stresses 
(e.g., heat-shock, light deprivation, etc.). 

Experience in genetic analysis of Arabidopsis (TDNA insertions, 
transgenics, gene silencing, over-expression) is required. 
Experience with DNA arrays is highly desirable.  The successful 
candidate must be able to demonstrate strong interpersonal skills 
(supervising and teaching technicians, graduate students and 
undergraduates), a willingness to learn and apply new techniques, the 
ability to design experiments independently, and strong writing 

Please send an application letter, including a CV, statement of 
interest and three letters of reference to Dr. Karen S. Browning, 
Dept. Chemistry and Biochemistry, University of Texas at Austin, 
Austin, TX  78712 or kbrowning at  See

The University of Texas at Austin is an Equal Opportunity Employer.

Dr. Karen S. Browning
Associate Professor
Department of Chemistry and Biochemistry
University of Texas
Austin, TX  78712

512-471-8696 FAX
kbrowning at ---

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