post-doctoral position available

Karen Browning kbrowning at mail.utexas.edu
Mon Aug 26 12:10:18 EST 2002



Postdoctoral Position
The University of Texas at Austin, Department of Chemistry and Biochemistry
and The Institute for Cellular and Molecular Biology

A postdoctoral position is available to study the role of the isozyme form
of eIF4F in plants.  This NSF funded postdoctoral position will include
biochemical analysis, insertion mutants, DNA arrays and gene silencing in
Arabidopsis thaliana.

The initiation of protein synthesis in all eukaryotes is an intricate
process requiring at least 8-10 known initiation factors.  The long-term
goal of this laboratory is to elucidate a detailed molecular description of
this process in plants.    Plant translation utilizes a second form of
eIF4F not found in other eukaryotes.  This isozyme form is a complex
composed of a cap-binding protein (eIF(iso)4E) and a large subunit
eIF(iso)4G.  The analysis of the roles of these two forms of eIF4F will
include the formation of mixed complexes of the subunits of eIF4F and
eIF(iso)4F (i.e., eIF4G/eIF(iso)4E and eIF(iso)4G/eIF4E).  A genomic
approach will also be taken that includes gene disruption of the subunits
using T-DNA knockouts or gene silencing.  A DNA chip will be prepared that
contains the genes for all the protein synthesis initiation, elongation and
termination factors from A. thaliana.  This chip will be probed with RNAs
obtained from various plant tissues, developmental stages and environmental
conditions to determine which genes for eIF(iso)4F or eIF4F, or other
protein synthesis factor genes, are regulated developmentally or during
environmental stresses (e.g., heat-shock, light deprivation, etc.).

Experience in genetic analysis of Arabidopsis (TDNA insertions,
transgenics, gene silencing, over-expression) is required.   Experience
with DNA arrays is highly desirable.  The successful candidate must be able
to demonstrate strong interpersonal skills (supervising and teaching
technicians, graduate students and undergraduates), a willingness to learn
and apply new techniques, the ability to design experiments independently,
and strong writing skills.

Please send an application letter, including a CV, statement of interest
and three letters of reference to Dr. Karen S. Browning, Dept. Chemistry
and Biochemistry, University of Texas at Austin, Austin, TX  78712 or
kbrowning at mail.utexas.edu.  See http://neon.cm.utexas.edu/browning/

The University of Texas at Austin is an Equal Opportunity Employer.

******************************************
Dr. Karen S. Browning
Associate Professor
Department of Chemistry and Biochemistry
University of Texas
Austin, TX  78712

512-471-4562
512-471-8696 FAX
http://www.cm.utexas.edu/browning/
kbrowning at mail.utexas.edu


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