RIKEN Arabidopsis Full-Length cDNAs (RAFL cDNAs)

Seki Motoaki mseki at gsc.riken.go.jp
Mon Dec 9 21:14:21 EST 2002


Dear Arabidopsis researchers,

Plant Functional Genomics Research Group of RIKEN Genomic Sciences Center
(GSC) (PI: Dr. Kazuo Shinozaki) of Japan is pleased to announce that 2055
RIKEN Arabidopsis Full-Length  (RAFL) cDNA clones whose full-length
sequences were determined will be publicly available from the RIKEN
Bioresource Center (PI: Dr. Masatomo Kobayashi,
e-mail:kobayasi at rtc.riken.go.jp) of Japan soon.

Collection and full-length sequencing of new 2055 RAFL cDNA clones were
carried out in the following ways:

    1.  Plant Functional Genomics Research Group of RIKEN GSC have
constructed Arabidopsis full-length cDNA libraries since 1996 (Seki et al.,
Plant J. 1998, 15: 707-720), isolated ca. 150,000 RAFL cDNA clones,
performed single-pass sequencing from both ends and clustered them in
collaboration with Genome Exploration Research Group of RIKEN GSC (PI: Dr.
Yoshihide Hayashizaki). They were clustered into 14,668 nonredundant RAFL
cDNA groups, about 60% of predicted genes in Arabidopsis (Seki et al.,
Science 2002, 296: 141-145).

    2.  Recently, Plant Functional Genomics Research Group of RIKEN GSC have
carried out the full-length sequencing and annotation of the 2055 new RAFL
cDNA clones.

    3.  Plant Functional Genomics Research Group  and Genome Exploration
Research Group of RIKEN GSC deposited the full-length sequence data of the
2055 RAFL cDNA clones to DDBJ/NCBI/EMBL public database and deposited the
RAFL cDNA clones whose full-length cDNA sequences had been determined  to
RIKEN Bioresource Center.

4. RIKEN Bioresource Center will then distribute the RAFL cDNA clones to
the academic researchers  soon.

Plant Functional Genomics Research Group of RIKEN GSC, and Arabidopsis SSP
Group of USA, which comprises  the Salk Institute (PI: Dr. Joseph R.
Ecker), Stanford Genome Technology Center (PI: Dr. Ronald W. Davis), and
Plant Gene Expression Center (PI: Dr. Athanasios Theologis) have determined
the full-length cDNA  sequences of the 10,107 RAFL cDNA clones  and
deposited the full-length sequence data of the 10,107 RAFL cDNA clones to
DDBJ/NCBI/EMBL public database as of Nov. 29, 2002. RIKEN Bioresource
Center  has distributed the 10,107 RAFL cDNA clones to the academic
researchers  from the end of April, 2002.

We hope that these RAFL cDNA clones will be useful resources for correct
annotation of genes on the genomic sequence and functional analysis of
genes and their products for academic researchers.

Best regards.

Motoaki Seki
Kazuo Shinozaki
Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center

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