harvesting RNA from Silique tissue
jredman at tigr.org
Wed Dec 11 15:35:23 EST 2002
<x-charset iso-8859-1>Good afternoon, everyone.
Recently I made a second attempt at harvesting total RNA from silique tissue
using TRIzol reagent and Invitrogen's protocol, followed by a polysaccharide
precipitation. Analysis indicates the only thing I was able to recover was
tRNA. Can anyone share tips or a better protocol for extracting total RNA
from samples such as siliques?
The Institute for Genomic Research
9712 Medical Center Dr.
Rockville, MD 20850
To have me paged at TIGR: 301-838-0200
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