harvesting RNA from Silique tissue

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Fri Dec 13 16:28:13 EST 2002


<x-flowed>I've found the method described in the paper by Chang et al. (1993)
"A simple and efficient method for isolating RNA from pine trees"
Plant Molecular Biology Reporter 11:113-116 to work well for a wide
variety of problematic plant tissues.

Mike

>
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>>    <x-charset iso-8859-1>Good afternoon, everyone.
>>
>>    Recently I made a second attempt at harvesting total RNA from
>>silique tissue
>>    using TRIzol reagent and Invitrogen's protocol, followed by a
>>polysaccharide
>>    precipitation.  Analysis indicates the only thing I was able to 
>>recover was
>>    tRNA.  Can anyone share tips or a better protocol for extracting total RNA
>>    from samples such as siliques?
>>
>>    I appreciate!
>>
>>    Julia
>>
>>    ____________________________________________
>>    Julia Redman
>>    The Institute for Genomic Research
>>    9712 Medical Center Dr.
>>    Rockville, MD 20850
>>
>>    To have me paged at TIGR:  301-838-0200
>>
>>    ---
>>
>>    </x-charset>
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-- 

Michael L. Sullivan, Ph.D
Research Plant Molecular
Geneticist
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax
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