BacterioMatch two-hybrid kit (Stratagene)

Joel Stafstrom t80jps1 at wpo.cso.niu.edu
Mon Jan 21 15:17:45 EST 2002


We're considering buying Stratagene's BacterioMatch two-hybrid kit.
We'd like to test for hypothesized interactions between proteins we're
studying.  In addition, we'd like to screen cDNA libraries for
additional protein binding partners.

1) Can you comment on your experience with this kit: successes,
failures, caveats?
2) Do you have an Arabidopsis cDNA library in the target vector (pTRG)
or know where one can be obtained?

Thanks in advance for your help,

Joel Stafstrom
stafstrom at niu.edu


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