GUS irregular expression pattern,,????????
ichoki at postech.ac.kr
Wed Mar 27 20:00:47 EST 2002
I have a problem,,,
If you know, teaches to me,,,, Its My major jobs..
My T-DNA is fussioned pCAMBIA3300(contain BAR) and pBI121(contain
and Transforamtion to the columbia. Then sowing basta selected T2
line.( sowing five allele for each lines)
21 days 3'-mature green leaf sampling(GL)
35 ~ 40 days 20% 4'-yellow leaf sampling(YL)
??? YL gus staining line is many(12%~16%), YL line number is
more than GL line number,
and many lines YL gus color is more deep blue then ML gus color.
and major problem is contain the partial spot- irregular type in same line.
I do't know. Why happened these types..
I guess some effect factors.
1.problem my sampling technic.
2.environmental effect in stress.(anthocyanin accmulation)
3.pCAMBIA backbone problem.
4.some artificial effect.
Good Luck to you..*^^*
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