BAC DNA isolation

James Meservy mesejl at
Tue May 28 16:40:46 EST 2002

I am trying to isolate BAC DNA in large quantity.  The intention is to
perform restriction digest of the BAC DNA for the eventual subcloning
and invivo complementation.  I have been remarkably unsucessful in
purifying any significant quantity of DNA.  Does anyone have a good
protocol or other useful suggestion.


More information about the Arab-gen mailing list