which is the best external control for RT-PCR

Chunxin Wang cxwang at wam.umd.edu
Tue Nov 12 10:30:47 EST 2002

Hi all,

We're currently using actin as the external control for RT-PCR. For some
reasons, we couldn't get stable results. It worked well sometimes and then
didn't work at all. Or it preferentially gets amplified in a certain
of tissues.

Any suggestions on which gene is a better control? How about tubulin,
ubiquitin and GAPDH?




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