Arabidopsis thaliana spy mutants

[Nobody]

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Dear All

I have the mutant of Arabidopsis thaliana (we call it spy 2) which 
has light-green leaves, high elongation of stem like constitutive 
GA-response mutant, spy-1. According to Jacobsen S.E., Olszewski N. 
E., 1993, spy-1 mutant is characterized by increasing resistance to 
inhibitor of GA biosynthesis, paclobutrazol (pcb), compared with 
ecotype Columbia.
However, when I compared the resistance of my mutant and spy-1 mutant 
to pcb, the resistance of spy-1 mutant was not differed from ecotype 
Columbia. I dissolved pcb  in ethanol, but authors dissolved pcb in 
acetone. I would like to know, if the differences in results could be 
due to the type of solvent?

Thanks you in advance

Sincerely yours,
Olga
PhD student Olga Sklyarova
Department of Genetics
Biological faculty
Moscow State University
119899 Moscow
Russia
Trinity2001 at newmail.ru


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