PCR from cell cultures in stress

alex levine alexl at mail.ls.huji.ac.il
Fri Oct 25 04:52:04 EST 2002


Did anyone encounter problems extracting DNA and/or doing PCR in
Arabidopsis (or other) cell cultures growing in high salt or iron
concentrations (or other stresses). I have problems getting DNA from
cells in iron. While in salt I get nice DNA but cannot PCR it.
many thanks
Alex


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