Northerns of RNAi lines

john jsmv at
Wed Aug 13 20:15:32 EST 2003

Just wondering whether other groups have found unsual results when
conducting Northerns on RNAi lines?  We are currently finding widespread
hybridisation in the form of several smearing bands (not due to
degradation of total RNA) in the RNAi lines.  Wild type RNA samples,
however,  produce sharp single bands of this gene as is expected.


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