DMSO as a cryoprotector

ani anidryan at
Thu Jun 5 22:15:01 EST 2003

<Dear colleagues,
we use DMSO (10% final conc) as cryoprotector to keep E. coli 
cultures ( our gene library) at -80 degree Celcius.
Please, tell me if you had that experience maintaining  the 
libraries- E. coli cultures - how many times I can freeze and defrost 
the cultures and they to be still viable?
Maybe the freezing-defrosting procedure  is also of importance  - 
slow or fast ?
Thank you,

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