DMSO as a cryoprotector
anidryan at videotron.ca
Thu Jun 5 22:15:01 EST 2003
we use DMSO (10% final conc) as cryoprotector to keep E. coli
cultures ( our gene library) at -80 degree Celcius.
Please, tell me if you had that experience maintaining the
libraries- E. coli cultures - how many times I can freeze and defrost
the cultures and they to be still viable?
Maybe the freezing-defrosting procedure is also of importance -
slow or fast ?
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