siliencing mulitiple genes by RNAi

[Shunyuan Xiao]

What about making a chimeric RNAi construct:  linking  unique sequences from
each of  3 or 4 or more target genes first and then making the inverted
repeat?   Theroetically this kind of construct should generate 100% matching
& unique small double stranded RNA effective in silencing the corresponding
genes.

Has anybody done this and got it work??


Shunyuan Xiao, PhD

School of Biological Sciences

University of East Anglia, Norwich  NR4 7TJ

United Kingdom





----- Original Message -----
From: ""Michael L. Sullivan"" <mlsulliv at facstaff.wisc.edu>
Newsgroups: bionet.genome.arabidopsis
To: <arab-gen at net.bio.net>
Sent: Monday, June 09, 2003 5:47 PM
Subject: Re: siliencing mulitiple genes by RNAi


>  <x-flowed> From my reading of the literature, I think it should be
possible
>  provided there is sufficient sequence identity among the genes.  The
>  figure 85% sticks out in my mind for plants, although I don't recall
>  which paper I got that from (perhaps from something from Peter
>  Waterhouse's lab).
>
>  Depending on how much similarity there is between the genes, you
>  might feel more or less confident about taking a "one construct"
>  approach.  If the similarity among genes were >90%, I'd be tempted to
>  just make one construct.  Less than that I might hedge my bets with
>  multiple constructs.  But first I'd scour the literature a bit more--
>  I think the data is out there.
>
>  Good luck.
>
>  Mike
>
>
>
>  >Has anyone any experience of silencing multiple genes with the same
>  >construct? Can it be done for 3 genes, or will I have to make three
>  >separate knockouts and cross them?
>  >
>  >Lee
>  >
>  >---
>
>
>  --
>
>  Michael L. Sullivan, Ph.D
>  Research Plant Molecular
>  Geneticist
>  U.S. Dairy Forage Research Center
>  1925 Linden Drive West
>  Madison WI, 53706
>
>  (608) 264-5397 Phone
>  (608) 264-5147 Fax
>  ---
>
>  </x-flowed>
>

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