siliencing mulitiple genes by RNAi
ricky_boernke at gmx.net
Fri Jun 27 04:47:41 EST 2003
We have fused together two sequences and both genes were targeted
with similar efficiency. More should also be possible.
s.xiao at uea.ac.uk ("Shunyuan Xiao") wrote in message
news:<001801c32f71$428fb4f0$0b41de8b at TURNERXP2>...
> What about making a chimeric RNAi construct: linking unique sequences from
> each of 3 or 4 or more target genes first and then making the inverted
> repeat? Theroetically this kind of construct should generate 100% matching
> & unique small double stranded RNA effective in silencing the corresponding
> Has anybody done this and got it work??
> Shunyuan Xiao, PhD
> School of Biological Sciences
> University of East Anglia, Norwich NR4 7TJ
> United Kingdom
> ----- Original Message -----
> From: ""Michael L. Sullivan"" <mlsulliv at facstaff.wisc.edu>
> Newsgroups: bionet.genome.arabidopsis
> To: <arab-gen at net.bio.net>
> Sent: Monday, June 09, 2003 5:47 PM
> Subject: Re: siliencing mulitiple genes by RNAi
> > <x-flowed> From my reading of the literature, I think it should be
> > provided there is sufficient sequence identity among the genes. The
> > figure 85% sticks out in my mind for plants, although I don't recall
> > which paper I got that from (perhaps from something from Peter
> > Waterhouse's lab).
> > Depending on how much similarity there is between the genes, you
> > might feel more or less confident about taking a "one construct"
> > approach. If the similarity among genes were >90%, I'd be tempted to
> > just make one construct. Less than that I might hedge my bets with
> > multiple constructs. But first I'd scour the literature a bit more--
> > I think the data is out there.
> > Good luck.
> > Mike
> > >Has anyone any experience of silencing multiple genes with the same
> > >construct? Can it be done for 3 genes, or will I have to make three
> > >separate knockouts and cross them?
> > >
> > >Lee
> > >
> > >---
> > --
> > Michael L. Sullivan, Ph.D
> > Research Plant Molecular
> > Geneticist
> > U.S. Dairy Forage Research Center
> > 1925 Linden Drive West
> > Madison WI, 53706
> > (608) 264-5397 Phone
> > (608) 264-5147 Fax
> > ---
> > </x-flowed>
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