leaf stomata staining

Alisa Ramakrishnan aramakris at yahoo.com
Fri May 9 17:48:21 EST 2003


Hi,

We want to use a fluorescent stain for stomata so we can estimate 
density on intact leaves of Piriqueta. I figured protocols may be 
similar to some used in Arabidopsis. Does anyone know of an easier 
way than vacuum infiltration to get the staining solution evenly 
distributed across the leaf surface despite trichomes and cuticle?

Thanks,

Alisa



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