anybody has tried to detect a protein using immunohistochemistry

pangju2 at snu.ac.kr pangju2 at snu.ac.kr
Tue May 13 03:59:58 EST 2003


hi,
I'm working on detecting a low abundant protein which is suspected to 
work as a transcription factor.
It's been faintly detected only in overexpression line and failed to 
detect in Col.

If there is anyone who tried to detect it using immunohistochemistry, 
please give me your valuable comments about what the better 2nd 
antibody conjugate is.
I read that using enzyme-linked 2nd antibody is better in sensitivity 
than using fluorescent dye conjugates.

please tell me your experiences.
thank you



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