translation of thio-mRNA (thioated mRNA)
naito at abs.agr.hokudai.ac.jp
Mon Nov 3 07:43:26 EST 2003
I have two questions:
1. Oligo RNAs containing phosphorothioate linkage, or thio-RNA, are
widely used in antisense oligo RNA strategy. One major reason is that
thio-RNAs are resistant to RNases.
Does any one know if thio-mRNAs are translatable in vitro in eukaryotic
systems such as wheat germ extract or reticulocyte lysate (or in vivo)?
There are papers reporting the translatability of thio-mRNAs in E. coli
extract (NAR, 19: 547-552, 1991; J. Biotechnol., 34: 61-69, 1994:
efficient translation is reported).
2. Nomenclature of phosphorothioate / phosphothioate.
In literatures and web sites, both phosphorothioate and phosphothioate
can be found. In some papers both words appear in the same paper.
At first, I thought that these discriminate
-O-P(=O)(-S*)-O- [* for a minus charge]
however, these two could be interchangeable.
OR,... does phosphothioate mean
Thanks for your suggestions.
Division of Applied Bioscience
Graduate School of Agriculture, Hokkaido University
Sapporo 060-8589, JAPAN
e-mail: naito at abs.agr.hokudai.ac.jp
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