translation of thio-mRNA (thioated mRNA)

Satoshi Naito naito at abs.agr.hokudai.ac.jp
Mon Nov 3 07:43:26 EST 2003


<x-flowed>Dear Friends,

I have two questions:

1. Oligo RNAs containing phosphorothioate linkage, or thio-RNA, are
widely used in antisense oligo RNA strategy.  One major reason is that
thio-RNAs are resistant to RNases.

Does any one know if thio-mRNAs are translatable in vitro in eukaryotic
systems such as wheat germ extract or reticulocyte lysate (or in vivo)?

There are papers reporting the translatability of thio-mRNAs in E. coli
extract (NAR, 19: 547-552, 1991; J. Biotechnol., 34: 61-69, 1994:
efficient translation is reported).

2. Nomenclature of phosphorothioate / phosphothioate.
In literatures and web sites, both phosphorothioate and phosphothioate
can be found.  In some papers both words appear in the same paper.

At first, I thought that these discriminate
-O-P(=O)(-S*)-O-   [* for a minus charge]
   and
-O-P(=S)(-O*)-O-
however, these two could be interchangeable.

OR,... does phosphothioate mean
-O-P(=O)(-O*)-S-
   or
-S-P(=O)(-O*)-O-

Thanks for your suggestions.
Satoshi Naito

=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
   Satoshi Naito
   Division of Applied Bioscience
   Graduate School of Agriculture, Hokkaido University
   Sapporo 060-8589, JAPAN
   Phone: +81-11-706-2800
   FAX: +81-11-706-4932
   e-mail: naito at abs.agr.hokudai.ac.jp
   http://arabi4.agr.hokudai.ac.jp/ArabiE/arabie.html
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=

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