Array quality RNA from Arabidopsis siliques

Gill Dean gilldean at interchange.ubc.ca
Fri Apr 30 12:28:33 EST 2004


Hi everyone,

I am currently trying to obtain RNA from developing Arabidopsis 
siliques for use in microarray. However, it seems that once the seeds 
in the siliques have completed mucilage production it is very hard to 
obtain RNA which can be labelled successfully. Mucilage consists of 
large amounts of pectin and I am assuming that this polysaccharide is 
what is causing the problem.

If anyone has a protocol which they have used for tissues with high 
polysaccharide, which gives RNA of array quality then I would be very 
grateful if you were willing to share!

Cheers,

Gill

Dr Gillian Dean
Post Doctoral Fellow
Dept of Botany
6270 University Boulevard
Vancouver
V6T 1Z4
Canada
Tel: (001) 604-822-2437
FAX: (001) 604 822-6089




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