Cell autonomy (CAUT) lines released by NASC

Graeme Gill graeme at arabidopsis.info
Fri Aug 6 09:23:13 EST 2004


<x-flowed>The Nottingham Arabidopsis Stock Centre (NASC) is pleased to announce
the release of the Cell Autonomy (CAUT) lines generated by Dr. Ian
Furner and co-workers at The Department of Genetics, University of
Cambridge, UK.

Cell-autonomy is a property of particular genotypes and is useful to
examine developmental and/or signalling interactions between cells and
tissues. Cell-autonomy is studied by producing genetic mosaics and
chimeras containing tissue of differing genotypes and analysing the
resulting phenotypes. A trait is completely cell-autonomous if the
genotype and phenotype of the tissue always correspond irrespective of
the genotype of the adjacent tissue. Conversely a trait is non
cell-autonomous if the phenotype of either tissue is affected by the
genotype of the adjacent tissue.

Cell-autonomy has been studied in plants using a variety of methods to
generate the chimeric or mosaic plants. Such methods include; grafting,
site-specific recombination, transposon excision and radiation induced
deletion. The latter method has been used for many elegant studies in
maize but in only one study of Arabidopsis (Furner et al 1996.
Development 122; 1041-1050). In these studies recessive cell-autonomous
colour markers are included in the experimental design to allow the
routine identification of the tissue containing the appropriate
deletion. This is comparatively easy in maize as many markers at
different locations are available. In Arabidopsis there is a shortage of
colour markers and finding a good cell-autonomous colour marker near a
gene of interest is not usually possible.

The CAUT lines are an attempt to overcome the shortage of useful markers
by artificially generating markers using genetic complementation and
Agrobacterium mediated transgenesis.
To order the lines please go to the NASC CAUT lines page -
http://seeds.nottingham.ac.uk/Nasc/go.lasso?page=browse/furner/caut.lasso

Here you will also find a comprehensive set of instructions including
the selection criteria and how to set up the experiment.

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