Microarray Workshop May 2004

[David Galbraith]

PLANT LONG OLIGONUCLEOTIDE MICROARRAY WORKSHOP

May 10-15, 2004
University of Arizona
Tucson, Arizona

This workshop will comprise a combination of lectures and hand-on 
laboratory sessions.  The participants will primarily employ 
Arabidopsis and maize whole genome 70-mer oligonucleotide microarrays 
in their laboratory work (for details of these arrays see 
http://www.ag.arizona.edu/microarray and http://www.maizearray.org/).

The workshop will be divided into two parts:  Part I  (Monday 4PM to 
Thursday 5PM) will cover wet-lab aspects of microarray target 
production, microarray hybridization, and scanning.  Part II (all 
day, Friday and Saturday) will concentrate on data extraction, 
statistical analysis, and experimental design.  Together these topics 
are aimed at the goal of obtaining optimal results using 
oligonucleotide-based microarrays.  Part II may be taken separately.

Specific topics to be covered include:

* Experimental design.
* Probe preparation and microarray printing.
* Microarray rehydration and probe immobilization.
* Target preparation, including RNA extraction, direct and indirect 
labeling, and amplification techniques.
* Microarray hybridization.
* Array scanning and data extraction.
* Data analysis and archiving.

Registration (Part I plus Part II) is $650, which includes costs of 
the Arabidopsis microarrays and other supplies that you will use. 
Part II registration only is $250.  Part I participants will be 
limited to 30 on a first-come, first-serve basis. Overall 
participation will be limited to 40 individuals.

For further details and to register, please contact
David Galbraith (galbraith at arizona.edu).

David W. Galbraith
University of Arizona
Department of Plant Sciences
303 Forbes Building
Tucson Arizona 85721
Tel: (520) 621-9153; Fax (520) 621-7186