tobacco leaf disk transformation: BASTA selection

[Benedikt Kost]

Please excuse a non-Arabidopsis question (I am having a hard time 
finding the info I am looking for in the literature):

I would appreciate very much if someone could let me know a selection 
scheme for BASTA resistance that works well with Agrobacterium 
mediated tobacco (N. tabacum SR1) leaf disk transformation (PPT vs 
BASTA vs ?, concentration levels that ensure tight selection without 
affecting regeneration).

Thank you very much for any help,

Benedikt Kost

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Benedikt Kost, PhD
Heidelberger Institute for Plant Sciences (HIP)
Im Neuenheimer Feld 360
69120 Heidelberg
Germany

+49 (0) 6221 54 5788 (tel)
+49 (0) 6221 54 5859 (fax)

<mailto:bkost at hip.uni-hd.de>bkost at hip.uni-hd.de

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