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[Arabidopsis] RNAi success and failure rates

Ian Furner ijf at mole.bio.cam.ac.uk
Mon Aug 22 06:47:38 EST 2005

Dear Detlef
                     I have some experience with RNAi using pHannibal. 
About 50% of plants containing a 700 Bp inverted repeat directed to the 
3' end of the CH-42 gene show some chlorophyl deficiency (CH-42 codes a 
sub-unit of Magnesum chelatase and is needed for chlorophyl 
production). I think you need to contact people who have a lot of 
experience  using hairpin constructs you might try Agrikola 
(http://www.agrikola.org/) or Chromodb (http://www.chromdb.org/).

Kind Regards Ian Furner

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