Thanks for those how responded already. I have been able to track down
all the pieces that I would need to make the Arabidopsis lines I want
but due to time constraints I was hoping to find someone who has already
constructed Arabidopsis lines co-expressing two FP variants targeting
different membranes - e.g. tonoplast-CFP and plasma membrane-GFP. This
way I could transform my construct (inducible expression of a gene that
causes a lethal phenotype from what I believe results from disrupted
membranes) into these lines and observe two membrane systems at the same
time and see which one is messed up first. I could look at each
membrane system individually in different lines and try and tell which
one is disrupted first but I think it would be much better if I could
observe them at the same time in the same cell. Thanks again.
Charles R. Dietrich, Ph.D.
Donald Danforth Plant Science Center
975 North Warson Road
St. Louis, MO 63132
Email: cdietrich at danforthcenter.org
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