Post-doctoral position on heme maturation in Paris

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Thu Mar 17 03:55:20 EST 2005

<x-flowed>Characterization of a new maturation pathway for c=92 heme biogene=

	Project description. The structure of=20
cytochrome b6f complex has revealed
the presence of a new heme ci bound by a unique thioether bond to
cytochrome b6. In photosynthesis this protein complex transfers electrons
from a liposoluble transporter to a hydrosoluble protein transporter while
building up a transmembrane proton gradient. Our genetic studies in the
unicellular green alga Chlamydomonas reinhardtii led to the identification
of 4 nuclear ccb loci that control maturation of heme ci (Kuras et al.
(1997) J. Biol. Chem. 272, 32427-32435; de Vitry et al. (2004),
Biochemistry 43, 3956-3968). We are currently identifying the first factors
of this new assembly pathway (system IV) that is fully distinct from those
previously known as controlling cytochrome c biogenesis through formation
of two thioether bonds. The project aims at an in depth characterization of
this new biogenesis pathway using two contrasting model organisms that
display ci-heme binding to their cytochrome bc/b6f complex. Arabidopsis
thaliana will be used to understand the molecular bases of the CCB pathway
in vascular plants through the analysis of insertion mutants in ccb
orthologues. The gram positive bacteria Bacillus subtilis is up to now the
only non photosynthetic organism in which a cytochrome bc complex with heme
ci has been biochimically characterized. The project includes the molecular
dissection of an operon that is comprised of a ccb orthologue and other
genes encoding putative additional factors. The project will start with the
biochimical and functional characterization of pre-existing mutants of
Arabidopsis and Bacillus.

	Competence. This post-doctoral position=20
is best suited for applicants that
hold a PhD degree with solid background in either biochimistry, molecular
biology or genetics and prior research experience with Arabidopsis. The
Bacillus subtilis work should benefit from the expertise of other groups in
our institute.

	Host Laboratory. The post-doctoral position is open at the Institut de
Biologie Physico-Chimique, Paris, France. It is funded by the CNRS for two
years (net salary about 1800 */month) starting September 1st 2005. The
post-doctoral associate will be working in the laboratory =93Membrane and
Molecular Physiology of Chloroplasts=94 of F.-A. Wollman where he/she will
join the team of Catherine de Vitry and Richard Kuras.

	Application should be sent to Catherine de Vitry (devitry at and
Richard Kuras (kuras at Candidates should supply a covering letter
describing their expertise and interests for the topic, a CV with a
publications list, and contact information with at least two referees.

	Dealine: applications should be sent before May 5th 2005

More Contacts: Catherine de Vitry & Richard Kuras, UMR 7141 (CNRS/UPMC),
Institut de Biologie Physico-Chimique, 13 Rue Pierre et Marie Curie, 75005
Paris, France.
Tel: 33 (0)1 58 41 50 55 de Vitry & 33 (0)1 58 41 50 50 Kuras; Fax: 33 (0)1
58 41 50 22
Email: devitry at & kuras at


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