[Arabidopsis] bar selection in cell cultures?
lentza at Cardiff.ac.uk
Mon Nov 21 04:56:31 EST 2005
I have a couple of plant transformation vectors containing the bar gene as
selectable marker. I would like to transform these vectors into cell cultures
(Arabidopsis and tobacco) and I don't know which compound (phosphoinothricin
(Basta), or methionine sulfoximine) and at which concentrations that would be
suitable to use as selection in cell cultures. Any advice will be appriciated.
Many thanks in advance.
Anne Lentz, MSc.
School of Biosciences - Cardiff University
PO Box 915
Cardiff, CF10 3TL
Email: LentzA at cf.ac.uk
Telephone: +44 (0) 297 5880 /
+44 (0) 297 6036
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