[Arabidopsis] how to screen out homozygous OE lines?

Y Jiang jiangyqcn at hotmail.com
Fri Aug 11 15:46:10 EST 2006

Arabidopsis researchers,

Could you tell me how to screen out homozygous overexpression lines among T2 
progenies using PCR? There is an endogenous gene in all T2 lines, and I need 
to differentiate hemizygous from homozygous lines?

Thanks for your input,


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