[Arabidopsis] Gentamycin selection

Y Jiang jiangyqcn at hotmail.com
Mon Aug 21 23:24:04 EST 2006


I once tried 50 ug/ml in 1/2 X MS; all seedlings growed very well. Then I 
increased to be 200 ug/ml, and it worked and only a few survived. Search the 
newsgroup for earlier posts on Gen dosage.

Good luck,

Jiang


>From: apurv <apurv at interchange.ubc.ca>
>To: arab-gen at magpie.bio.indiana.edu
>Subject: [Arabidopsis] Gentamycin selection
>Date: Thu, 17 Aug 2006 17:23:14 -0700 (PDT)
>
>Hello Researchers,
>I am using a plant transformation vector that has gentamycin selection. I 
>tried using 50 mg/L selection and it did not work (everything was growing 
>happily). Can you suggest the maximum/appropriate concentration of 
>gentamycin in the media to select the transgenics?
>Thanks,
>Apurv
>
>
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