[Arabidopsis] screening TAC library
(by gilldean At interchange.ubc.ca)
Fri Dec 1 19:42:28 EST 2006
I am interested in screening the TAC library available from the ABCR for TACs which cover the region containing a locus that we are map-based cloning. The TAC libray is spotted onto a filter and I am planning to screen using 32P-labelled probes.
Does anyone have experience in designing such a probe set and carrying out the filter hybridisation using a mixture of probes for this TAC library (or something similar)? The average insert size of each TAC is 80 kb and our region of interest is 200 kb.
Thanks a lot for your help,
Dr Gillian Dean, Haughn Lab
Dept of Botany
6270 University Boulevard
Tel: (001) 604-822-2437
FAX: (001) 604 822-6089
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