[Arabidopsis] screening TAC library

[Gill Dean]

Hi everyone,

I am interested in screening the TAC library available from the ABCR for TACs which cover the region containing a locus that we are map-based cloning. The TAC libray is spotted onto a filter and I am planning to screen using 32P-labelled probes.

Does anyone have experience in designing such a probe set and carrying out the filter hybridisation using a mixture of probes for this TAC library (or something similar)? The average insert size of each TAC is 80 kb and our region of interest is 200 kb.

Thanks a lot for your help,

Cheers,

Gill

Dr Gillian Dean, Haughn Lab
Dept of Botany
6270 University Boulevard
Vancouver
V6T 1Z4
Canada
Tel: (001) 604-822-2437
FAX: (001) 604 822-6089
http://www3.botany.ubc.ca/haughn/