[Arabidopsis] PCR

savita At unt.edu via arab-gen%40net.bio.net (by savita At unt.edu)
Tue Dec 5 21:41:28 EST 2006


Hi

Will a PCR not be as efficient if the annealing temperature is higher 
(i.e. above 80C) than the polymerization temperature? What needs to be 
done in such a case to make the reaction work?

Thanks
Savita



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