[Arabidopsis] Re: screening TAC library

[Leah.Clissold At gmail.com]

Dear Gill.

I work in the Genome Laboratory here at The John Innes Centre in
Norwich and was wondering if you required any information about the
libraries we hold here.  I noticed that you may be interested in the
JAtY library for in-silico mapping.

If you do require any more help you can contact me on
leah.clissold At bbsrc.ac.uk.

Many Thanks

Leah

On Dec 1, 7:42 pm, Gill Dean <gilld... At interchange.ubc.ca> wrote:
> Hi everyone,
>
> I am interested in screening the TAC library available from the ABCR for TACs which cover the region containing a locus that we are map-based cloning. The TAC libray is spotted onto a filter and I am planning to screen using 32P-labelled probes.
>
> Does anyone have experience in designing such a probe set and carrying out the filter hybridisation using a mixture of probes for this TAC library (or something similar)? The average insert size of each TAC is 80 kb and our region of interest is 200 kb.
>
> Thanks a lot for your help,
>
> Cheers,
>
> Gill
>
> Dr Gillian Dean, Haughn Lab
> Dept of Botany
> 6270 University Boulevard
> Vancouver
> V6T 1Z4
> Canada
> Tel: (001) 604-822-2437
> FAX: (001) 604 822-6089http://www3.botany.ubc.ca/haughn/