[Arabidopsis] Use of segregating WT as control

Arul Varma arulvar at gmail.com
Tue Jan 10 09:46:35 EST 2006


I was wondering why not use the segregating Wild-Type from an heterozygous
plant as the wild-type-control, if it has very clear distinguishing features
from the +/- or the -/-, for any type of  quantification expts like the
RT-PCR or qPCR, since the seed-lot is exactly the same as that of the mutant
being studied?

Is there any caviar behind this theory that I am not aware of? Thanks for
your inputs.

Arul


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