[Arabidopsis] Auxin overproduction in iaaH transformants

[Danielle Marcos]

Hello,

I'm currently working with Arabidopsis Col-0 plants transformed with an 
inducible indoleacetamide hydrolase (iaaH) transgene.  The iaaH gene 
encodes an enzyme that catalyzes the production of auxin (in the form 
of indole acetic acid or IAA) from indoleacetamide (IAM).  Col-0 plants 
do not produce IAM. Therefore, only plants expressing iaaH that are 
supplied with IAM should overproduce auxin, while plants expressing 
iaaH that are not supplied with IAM should not.  However, I observe 
phenotypes consistent with auxin overproduction in plants expressing 
iaaH both in the presence and absence of auxin.  Has anyone else had a 
similar experience?  Is there any way of avoiding this?

Thanks,

Danielle Marcos