[Arabidopsis] protoplast from arabidopsis- HELP

galonyae at post.tau.ac.il galonyae at post.tau.ac.il
Mon May 29 05:48:14 EST 2006

I have a few questions about the procedure:

Is it necessary to grow plants on sterile medium or growth room is good enough?

Is  wounding with a razor a critical step?

There are many companies that supply Manitol, which one is the best?
Also the digesting enzymes... I need a recomendation.

which density of protoplasts is the best to do transient transformation to?

thanks in advance:)), Yael

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