[Arabidopsis] Re: MS Plate Contamination (Heather Babka)

Morgan Xu via arab-gen%40net.bio.net (by xu.161 from osu.edu)
Thu Apr 26 12:11:36 EST 2007


   500ug/ml Carbenicillin or 200ug/ml Cefotaxin in the MS plate will kill
   the agrobacteria, without harming the plants.
   
   Good luck!
   
   
   Morgan
   
   ----- Original Message -----
   
   From: arab-gen-request from oat.bio.indiana.edu
   
   Date: Thursday, April 26, 2007 1:01 pm
   
   Subject: Arab-gen Digest, Vol 24, Issue 16
   
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   > Today's Topics:
   >
   > 1. MS Plate Contamination (Heather Babka)
   > 2. POSTDOCTORAL POSITION (Sophia Stone)
   >
   >
   > -------------------------------------------------------------------
   > ---
   >
   > Message: 1
   > Date: Tue, 24 Apr 2007 16:40:37 -0500
   > From: "Heather Babka" <hbabka from iastate.edu>
   > Subject: [Arabidopsis] MS Plate Contamination
   > To: arab-gen from magpie.bio.indiana.edu
   > Message-ID:
   > <479139e20704241440h3e3c7589u29620ec0cceeaa64 from mail.gmail.com>
   > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
   >
   > Hello,
   >
   > I am trying to select T1 plants on MS plates with 30ug/mL
   > Hygromycin. It
   > seems that all of this newly collected seed is contaminated with
   > bacteria(Agro?). Is there anything I can add to the media that
   > would kill the
   > Agrobacteria, but not harm the plants? Currently I sterilize with
   50%
   > Bleach + 0.02% Triton. I tried adding a 70% Ethanol wash but that
   > did not
   > help.
   >
   > Thanks,
   > Heather
   >
   > --
   > Heather L. Babka
   > Interdepartmental Genetics
   > Department of Genetics, Development, and Cell Biology
   > 433 Bessey Hall
   > Iowa State University
   > Ames, IA 50011
   > Phone: (515) 294-3509
   > Fax: (515) 294-1337
   >
   >
   > ------------------------------
   >
   > Message: 2
   > Date: Wed, 25 Apr 2007 11:57:26 -0300
   > From: Sophia Stone <Sophia.Stone from DAL.CA>
   > Subject: [Arabidopsis] POSTDOCTORAL POSITION
   > To: arab-gen from magpie.bio.indiana.edu
   > Message-ID: <20070425115726.40d3bhssgiskgcoc from my1.dal.ca>
   > Content-Type: text/plain; charset=ISO-8859-1
   >
   > POSTDOCTORAL POSITION
   > Dalhousie University, Halifax, NS Canada
   >
   > A postdoctoral position is available starting September 2007 to
   > study the
   > biochemistry and molecular biology of the ubiquitination
   > proteasome pathway in
   > Arabidopsis thaliana. Successful applicants will join a new
   > research group in
   > the Biology department at Dalhousie University currently using a
   > variety of
   > techniques to define the pathway's role in plant development and
   > response to
   > stress. Candidates must have a strong background in protein
   > biochemistry and
   > molecular biology techniques. Previous experience in working with
   > Arabidopsisis advantageous but not required. Salary will be
   > commensurate with experience.
   > Interested persons should send a CV and three letters of
   > recommendation to Dr.
   > Sophia Stone, Department of Biology, Dalhousie University, 1355
   > Oxford Street
   > Halifax, NS Canada B3H 4J1; email- Sophia.Stone from dal.ca or contact
   > her at
   > 902-494-4541 (http://biology.dal.ca/us/f/stone/stone.html).
   >
   >
   > Sophia Stone
   > Assistant Professor
   > Department of Biology
   > Life Science Centre
   > Dalhousie University
   > 1355 Oxford Street
   > Halifax, Nova Scotia
   > Canada B3H 4J1
   > Email: Sophia.Stone from dal.ca
   > Phone: 902-494-4541
   > Fax: 902-494-3736
   >
   >
   >
   > ------------------------------
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