[Arabidopsis] RNA extraction from etiolated seedlings

[Fankhauser Christian]

   Hi Stefan,
   you can obtain high quality RNA from etiolated seedlings using Qiagen
   columns. The yield is low particularly if you grow your seedlings in
   the absence of sucrose (e.g. 1/2X MS is what we typically do). Don't
   grow your seedlings for more than 4-5 days (5 days is really the
   maximum, I prefer 3-4 days)
   in our hands what works best is to extremely quickly (under safe green
   light) harvest and grind the etiolated seedlings under liquid N2 using
   a mortar and pestle (pre-chilled using liquid N2). This fine powder
   can then be stored at -70°C for short periods of time, we get best
   results by immediately processing it to get RNA.
   good luck   Christian
   
   --
   
   Christian Fankhauser
   
   CIG
   
   Genopode Building
   
   University of Lausanne
   
   CH-1015 Lausanne
   
   
   Phone ++41 21 692 3941
   
               ++41 21 692 3920 (Nathalie Clerc, administrative
   assistant)
   
   FAX    ++41 21 692 3925
   
   
   [1]http://www.unil.ch/cig/page8391_en.html

References

   1. http://www.unil.ch/cig/page8391_en.html