[Arabidopsis] about immunoprecipitation method for basic proteins

chenxi via arab-gen%40net.bio.net (by cxde At iastate.edu)
Thu Jan 25 21:42:03 EST 2007


Hello colleagues,

Sorry to bother you. I can not pull down the specific basic proteins, when 
using immunoprecipitation for Arabidopsis. The method I used is below:
Firstly, I used high/low salt solution to lyse the nuclei from the leaf 
tissue, then do the dialysis to get rid of the excessive salt. Finally, I 
incubated this extracts with the complex: G-protein+specific basic protein 
antibody. However, the western-blot results showed that the antibody can not 
pull down its own specific basic protein. It means no positive signal appears.
the antibody is fine. I don't know what things happen to result in this. If 
you have some suggestion or advice, please feel free to contact with me. 
Thanks a lot!

Best regards!

Xi Chen





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