[Arabidopsis] RNA extraction from etiolated seedlings

[Stefan Weinl]

We are in a process to built our own cDNA library and therefore we are 
trying to isolate RNA from etiolated seedlings. Up to now we tried 
column-based kits (Qiagen and Promega) and used TriFast solution to 
isolate RNA but did not receive suitable RNA.
Now we would like to use a classical protocol using phenol and LiCl. We 
would be very thankful for a protocol which works for etiolated 
seedlings, or any suggestions regarding RNA extraction from etiolated 
material.

Thank you in advance,

Stefan