IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Arabidopsis] RE: RNA extraction from etiolated seedlingsArab-gen Digest, Vol 27, Issue 9

Wang XW via arab-gen%40net.bio.net (by X.W.Wang from rhul.ac.uk)
Wed Jul 18 12:33:11 EST 2007


Dear Stefan,

I also used the same kit (qiagen) and from same etiolated seedlings. And
It works well well.

I suggest you check:

1. low teperature extraction?
2.DEPC -treated water
3.exact seedlings (weight) the Kit required



Mr Xuewen Wang (PhD)
 
School of Biology
 Royal Holloway, Uni of London
Surrey UK
TW20 0EX
email: x.w.wang from rhul.ac.uk
 
 
 


-----Original Message-----
From: arab-gen-bounces from oat.bio.indiana.edu
[mailto:arab-gen-bounces from oat.bio.indiana.edu] On Behalf Of
arab-gen-request from oat.bio.indiana.edu
Sent: 18 July 2007 18:00
To: arab-gen from magpie.bio.indiana.edu
Subject: Arab-gen Digest, Vol 27, Issue 9


Send Arab-gen mailing list submissions to
	arab-gen from net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
	http://www.bio.net/biomail/listinfo/arab-gen
or, via email, send a message with subject or body 'help' to
	arab-gen-request from net.bio.net

You can reach the person managing the list at
	arab-gen-owner from net.bio.net

When replying, please edit your Subject line so it is more specific than
"Re: Contents of Arab-gen digest..."


Today's Topics:

   1. RNA extraction from etiolated seedlings (Stefan Weinl)


----------------------------------------------------------------------

Message: 1
Date: Tue, 17 Jul 2007 11:05:45 +0200
From: Stefan Weinl <Stefan.Weinl from online.de>
Subject: [Arabidopsis] RNA extraction from etiolated seedlings
To: arab-gen from magpie.bio.indiana.edu
Message-ID: <469C8669.1010901 from online.de>
Content-Type: text/plain; charset=ISO-8859-15; format=flowed

We are in a process to built our own cDNA library and therefore we are 
trying to isolate RNA from etiolated seedlings. Up to now we tried 
column-based kits (Qiagen and Promega) and used TriFast solution to 
isolate RNA but did not receive suitable RNA.
Now we would like to use a classical protocol using phenol and LiCl. We 
would be very thankful for a protocol which works for etiolated 
seedlings, or any suggestions regarding RNA extraction from etiolated 
material.

Thank you in advance,

Stefan




------------------------------

_______________________________________________
Arab-gen mailing list
Arab-gen from net.bio.net http://www.bio.net/biomail/listinfo/arab-gen

End of Arab-gen Digest, Vol 27, Issue 9
***************************************



More information about the Arab-gen mailing list

Send comments to us at biosci-help [At] net.bio.net