[Arabidopsis] Chloroplast RNA

Byung-Hoon Kim via arab-gen%40net.bio.net (by bkim2 from utk.edu)
Mon Jul 30 13:50:42 EST 2007

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Hi all,
I'm looking for the most simple and efficient way to label the chloroplast 
RNA.
I think, 5' end-labeling of chloroplast RNA by polynucleotide kinase will be 
straightforward. But I'm wondering about the 5' modification of chloroplast 
RNA. Is it somehow modified? Or do they have just 5' phosphate that can be 
easily dephosphorylated by alkaline phosphatase?
Any comment will be greatly appreciated.

Regards,
Byung-hoon Kim

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