[Arabidopsis] Extraction of sillique RNA of Arabidopsis?

Ben Holt via arab-gen%40net.bio.net (by benholt from ou.edu)
Sun Jun 3 21:40:20 EST 2007

Attached is a protocol for the isolation of RNA from siliques that I got 
in 2003 from Dr. Tamara Western (Assistant Prof. at McGill University I 
believe). I saved it all these years because I knew I would need it 
someday - well, I haven't needed it yet, but it sounds like others might!

Good Luck,
Ben Holt

Guan Yuefeng wrote:

> Hi everyone:
> I recently suffered problem with RNA extraction of silliques
> (including seeds)for RT-PCR.We have tried Trizol of Invitrogen and
> cold-phenol protocol but neither could get high quality RNA.Does
> anybody have protocol for RNA extraction of silliques?ANy suggestion
> will be appreciated.
> Shanghai China
>                                        Guan Yuefeng
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Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
GLCH Rm 219
770 Van Vleet Oval
Norman, OK  73019
Phone (405)325-9018
FAX   (405)325-7619

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