[Arabidopsis] 35S promoter and native promoter
(by gaoxinyou3 from yahoo.com)
Sat May 5 17:36:36 EST 2007
I am working on A gene in Arabidopsis thaliana. The A gene mutated plant is much short than WT plants, then transformed the A gene into the plants with 2 constructs:
Construct 1 = 35S promoter + A gene cDNA sequence,
Construct 2 = A gene promoter + A gene genomic DNA sequence
The phenotype was completely rescued by construct 2 transformation, but only partially rescued by construct 1 transformation.
I think the 35S promometr can not make A gene protein in the right places or enough amount.
Are they any other possibilities?
Please email me the other possibilities if you think it may have!
Thank you very much!
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