[Arabidopsis] Re: 35S promoter and native promoter

[asdunlap from gmail.com]

Did you sequence the mutation?  Have you considered the possibility of
aberrant splicing?

Amy
University of Illinois

On May 5, 5:36 pm, xinyou gao <gaoxiny... from yahoo.com> wrote:
> Dear all,
>   I am working on A gene in Arabidopsis thaliana. The A gene mutated plant is much short than WT plants, then transformed the A gene into the plants with 2 constructs:
>   Construct 1 = 35S promoter + A gene cDNA sequence,
>   Construct 2 = A gene promoter + A gene genomic DNA sequence
>
>   The phenotype was completely rescued by construct 2 transformation, but only partially rescued by construct 1 transformation.
>
>   I think the 35S promometr can not make A gene protein in the right places or enough amount.
>
>   Are they any other possibilities?
>   Please email me the other possibilities if you think it may have!
>   Thank you very much!
>   Gao
>   California, USA
>
> ---------------------------------
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