[Arabidopsis] RE: Marker for Columbia and C24 Ecotype

Nicholas Provart via arab-gen%40net.bio.net (by nicholas.provart from utoronto.ca)
Wed Sep 19 12:56:18 EST 2007


Hi Behailu,

The Bio-Array Resource (www.bar.utoronto.ca) has predicted all possible CAPS
markers (sets of PCR primers/enzyme combinations) for 96 ecotypes (Col-0 and
C24 included) based on Magnus Nordborg's 2005 partial sequence data set
across 96 accessions. These have not been verified in the lab, but in
general at least 75-80% of them work, based on our experience.

http://bar.utoronto.ca/markertracker/search.spy 

There seem to be several markers for Col-0 versus C24, at least for the
genomic regions I searched. Click the DETAILED VIEW on the output page to
see how the markers would run on virtual gels. 

Best,

Nick
.........................................
Nicholas Provart, PhD
Assistant Professor, Plant Bioinformatics
Director, Program in Genome Biology and Bioinformatics
Member, Centre for the Analysis of Genome Evolution and Function
Rm 3051, Dept. of Cell and Systems Biology, Uni. Toronto
25 Willcocks St., Toronto, ON. M5S 3B2. CANADA

Tel. (+1) (416) 978-7141, Fax. (+1) (416) 978-5878
URL. http://www.csb.utoronto.ca/faculty/provart/
Arabidopsis Tools. http://www.bar.utoronto.ca   
email. nicholas.provart from utoronto.ca
TTC. Spadina LRT, Willcocks St.




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