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[Arabidopsis] Can un-fused GFP enter the mitochondria?

dmolino from versailles.inra.fr via arab-gen%40net.bio.net (by dmolino from versailles.inra.fr)
Mon Aug 4 04:48:50 EST 2008


Salut Simon,
Absolutely not, a signal is needed to enter the mitocondria.
But if for some reasons your mitocondria get sick... you could see some
artefacts.
Selon simonmi1 from post.tau.ac.il:
Diana
> Dear all,
> it is common knowledge that the fluorescence of over-expressed,
> un-fused GFP (35S::GFP) can be easily detected in the cytoplasm and
> nucleus of plant cells. Does anyone know whether this free GFP enters
> the mitochondria as well?
> Thanks for your response is advance,
> Simon
>
>
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Diana Molino
Laboratoire de Biologie Cellulaire
INRA Versailles
Route de St-Cyr (RD10)
F-78026 VERSAILLES Cedex
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