[Arabidopsis] Re: Expression of a fusionprotein

zhouxin168 via arab-gen%40net.bio.net (by zhouxin168 from gmail.com)
Sun Aug 17 00:05:36 EST 2008


On Aug 15, 7:10 pm, Madlen Schiller <Madlen.Schil... from mailbox.tu-
dresden.de> wrote:
> Hello,
>
> at the moment I try to verify a fusion protein in Arabidopsis. I have  
> cloned the gen by using Gateway cloning (Invitrogen). I know that the  
> DNA-sequence is right and integrated in the Arabidopsis genom. But  
> unfortunately I get not transkripts (no RNA or Protein) in the  
> T1-generation.
> Does anyone has/had also trouble with the expression of fusion  
> proteins. I read that it is possible that the transcript will be done  
> in further generations? Or is it possible the the Gateway cloning  
> system decreased the expression?
>
> Thanks a lot,
> Madlen

could you dectect the fusion DNA insertion in those T1-generation
lines?



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